EC Number   |
Protein Variants |
Reference |
|---|
   2.4.1.90 | C134S |
complete loss of activity |
489552 |
| 2.4.1.90 | C342S |
33fold increase in the apparent Km-value for UDPgalactose |
489552 |
| 2.4.1.90 | D254E |
0.01% of the activity of the wild-type enzyme |
489556 |
| 2.4.1.90 | D254N |
0.01% of the activity of the wild-type enzyme |
489556 |
| 2.4.1.90 | D315A |
the mutant shows about 90% of wild type activity |
758173 |
| 2.4.1.90 | D320A |
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme |
489556 |
| 2.4.1.90 | D320E |
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme |
489556 |
| 2.4.1.90 | D320N |
when partially activated by Mn2+ binding to the primary site, can be further activated by Co2+ or inhibited by Ca2+, an effect that is the opposite of what is observed with the wild-type enzyme |
489556 |
| 2.4.1.90 | DELTA1-70 |
crystallization of a refolded Drosophila Cd7 protein coding the catalytic domain (Cd7) sequence (residues 71-322) is not successful |
719863 |
| 2.4.1.90 | DELTA1-70/DELTA312-322 |
a DNA fragment bearing the Cd7 catalytic domain and C-terminal 11-amino acid deletion (Cd7DELTAC) sequence (residues 71-311) shows no difference in catalytic activity or folding but crystalization is not possible |
719863 |
