EC Number |
Protein Variants |
Reference |
---|
2.4.1.62 | D175A |
overexpression of the mutant and -D291A does not rescue galactosylation deficiency of a knockout strain |
757755 |
2.4.1.62 | D176A |
overexpression of the mutant and -D291A does not rescue galactosylation deficiency of a knockout strain |
757755 |
2.4.1.62 | D177A |
overexpression of the mutant and -D291A does not rescue galactosylation deficiency of a knockout strain |
757755 |
2.4.1.62 | DELTA20C |
mutant with deletion of 20 amino acids from carboxy terminus + C-terminal MalE fusion, specific activity (mU/mg): 58.1 (substrate: GalNAcalpha), 127.5 (substrate: GalNAcbeta), 17.5 (substrate: GM2), 374 (substrate: GA2) |
692522 |
2.4.1.62 | DELTA20N |
mutant with deletion of 20 amino acids from carboxy terminus + N-terminal MalE fusion, specific activity (mU/mg): 3.3 (substrate: GalNAcalpha), 8.0 (substrate: GalNAcbeta), 2.3 (substrate: GM2), 34.4 (substrate: GA2) |
692522 |
2.4.1.62 | DELTA30C |
mutant with deletion of 30 amino acids from carboxy terminus + C-terminal MalE fusion, specific activity (mU/mg): 11.6 (substrate: GalNAcalpha), 75.7 (substrate: GalNAcbeta), 80.9 (substrate: GM2) |
692522 |
2.4.1.62 | DELTA30C |
mutant with deletion of 30 amino acids from carboxy terminus + C-terminal MalE fusion, specific activity (mU/mg): 531.5 (substrate: GalNAcalpha), 1299.5 (substrate: GalNAcbeta), 2420 (substrate: GM2), 271.5 (substrate: IFNalpha2b). kcat (1/sec): 0.5 (GalNAcalpha-FCHASE), 1.07 (GalNAcbeta-FCHASE), 0.05 (UDP-Gal), Km (mM): 1.68 (GalNAcalpha-FCHASE), 3.18 (GalNAcbeta-FCHASE), 1.11 (UDP-Gal) |
692522 |
2.4.1.62 | DELTA30N |
mutant with deletion of 30 amino acids from carboxy terminus + N-terminal MalE fusion, specific activity (mU/mg): 0.9 (substrate: GalNAcalpha), 5.3 (substrate: GalNAcbeta), 14 (substrate: GM2) |
692522 |
2.4.1.62 | DELTA30N |
mutant with deletion of 30 amino acids from carboxy terminus + N-terminal MalE fusion, specific activity (mU/mg): 49.5 (substrate: GalNAcalpha), 36 (substrate: GalNAcbeta), 96 (substrate: GM2), 14 (substrate: IFNalpha2b) |
692522 |
2.4.1.62 | more |
Cgt-/-, Cgt deficient mice, no accumulation of galactolipids, increased ceramide levels |
703880 |