EC Number |
Protein Variants |
Reference |
---|
2.4.1.243 | more |
exchange of N-terminal 36 amino acids with those of sucrose:sucrose 1-fructosyltransferase and vacuolar invertase. The latter two enzyme no longer used sucrose as a substrate, but were able to use 1-kestose |
666603 |
2.4.1.243 | N340D/W343R |
the mutant shows 6G-FFT and 1-FFT activities that hold the middle between those of the N340D and W343R single mutants |
706297 |
2.4.1.243 | N84A |
preferred addition of fructose to a terminal fructose instead of terminal glucose, producing inulin-type fructans |
666611 |
2.4.1.243 | N84A/S87N |
prefers synthesis of beta(2-1) linkages |
666603 |
2.4.1.243 | N84G |
no enzymic activity |
666611 |
2.4.1.243 | N84Q |
preferred addition of fructose to a terminal fructose instead of terminal glucose, producing inulin-type fructans |
666611 |
2.4.1.243 | N84S |
preferred addition of fructose to a terminal fructose instead of terminal glucose, producing inulin-type fructans |
666611 |
2.4.1.243 | N84Y |
no enzymic activity |
666611 |
2.4.1.243 | S415N |
the mutant shows slightly lower 6G-FFT/1-FFT activities, but the ratio between the main reaction products is not significantly affected |
706297 |
2.4.1.243 | S87D |
no change in substrate or product specificity |
666611 |