EC Number |
Protein Variants |
Reference |
---|
2.4.1.231 | D379N |
ratio of phosphorolysis to hydrolysis decreases by a factor of 287500 |
-, 671872 |
2.4.1.231 | H403A |
ratio of phosphorolysis to hydrolysis decreases by a factor of 2190 |
-, 671872 |
2.4.1.231 | K512A |
kcat/Km (phosphate): 0.0014/Msec, vanadate shows a weaker inhibitory effect compared to wild-type, propargylamine leads to a 23fold increase in activity, phosphate 50 mM enhances basal hydrolase activity |
692323 |
2.4.1.231 | K512A |
ratio of phosphorolysis to hydrolysis decreases by a factor of 511111 |
-, 671872 |
2.4.1.231 | R507A |
Km (phosphate) 103 mM, kcat/Km (phosphate): 0.0004/Msec, vanadate shows no inhibitory effect, guanidine leads to a 45fold increase in activity |
692323 |
2.4.1.231 | R507A |
ratio of phosphorolysis to hydrolysis decreases by a factor of 575000 |
-, 671872 |