EC Number   |
Protein Variants |
Reference |
|---|
   2.4.1.183 | more |
construction of several mutant strains in Aspergillus nidulans by gene replacement: agsA disruption, agsB disruption, and double-disruption strains, as well asl CagsB strains in which agsB expression is controlled by the inducible alcA promoter, with or without the agsA-disrupting mutation. The agsA disruption strains do not show markedly different phenotypes from the wild-type strain. The agsB disruption strains forms dispersed hyphal cells under liquid culture conditions, regardless of the agsA genetic background. Dispersed hyphal cells are also observed in liquid culture of the CagsB strains when agsB expression is repressed, whereas these strains grow normally in plate culture even under the agsB-repressed conditions. Phenotypes, overview |
-, 737101 |
| 2.4.1.183 | more |
deletion of all the three AGS genes, the ags1DELTAags2DELTAags3DELTA (agsDELTA) mutants are less virulent than the parental strain in murine model of aspergillosis, mutant phenotypes, overview. Susceptibility of the agsDELTA and parental strain conidia to antifungal molecules is similar. The resting conidia of the agsDELTA mutants are immediately recognized by the innate immune system of mice because the surface rodlet layer is masked by a layer of glycoproteins, overview |
737151 |
| 2.4.1.183 | more |
deltaags3, with a non-functional, disrupted AGS3 gene. The cell wall composition of the mycelium or the conidia of the deltaags3 mutant and the wild type strain does not present any qualitive or quantitive differences. The dense outer layer surrounding the conidial cell wall that contains melanin is 2times thicker in the deltaags3 mutants than in the wild type. Deletion of AGS1 results in a 2.8 and 1.9fold increase in the expression of AGS1 and AGS2, respectively. Lung invasion in infected mice is significantly higher in the mutant than in the wild type. The mutant has a better resistance to reactive oxygen species and a quicker germination rate |
673818 |
| 2.4.1.183 | more |
disruption of gene agsE in Aspergillus luchuensis (DELTAagsE) with Agrobacterium-mediated transformation (AMT) . The DELTAagsE protoplasts are also competent for transformation with the protoplast-PEG method. Phenotypes of the agsE disruptant, overview |
759525 |
| 2.4.1.183 | more |
disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. Mutant phenotypes, overview. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively |
-, 758930 |
| 2.4.1.183 | more |
disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. Mutant phenotypes, overview. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively |
-, 758930 |
| 2.4.1.183 | more |
disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively |
-, 758930 |
| 2.4.1.183 | more |
generation of an Aspergillus nidulans alpha-1,3-glucan in constitutive overexpression strain. Constitutive high-level alpha-1,3-glucan synthase activity increases hyphal wall thickness, but colonies grow slowly and sporulate poorly and have much higher adhesion to hydrophobic materials. In contrast to the wild-type strain, the overexpression strain forms a biofilm-like structure in plastic culture wells that is as adhesive as wild-type Aspergillus fumigatus. The high level of agsB expression is deleterious. Phenotype, overview |
759714 |
| 2.4.1.183 | more |
overexpression of agsA in Aspergillus nidulans under the control of a constitutive promoter in the genetic background of agsB disruptants, the native promoter is replaced by the constitutive tef1 promoter. Alkali-soluble glucan from the agsA overexpressing strain is composed mainly of alpha-1,3-glucan. The agsA gene is disrupted by using the Cre/loxP marker recycling system. Generation of an agsA deletion mutant strain agsADELTA. Genetic structure of mutant strains, overview. The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan |
-, 759245 |
| 2.4.1.183 | more |
overexpression of agsB in Aspergillus nidulans under control of a constitutive promoter in the genetic background of agsA disruptants, the native promoter is replaced by the constitutive tef1 promoter. Generation of an agsB deletion mutant strain agsBDELTA. Genetic structure of mutant strains, overview. The double mutant DELTAagsA-DELTAagsB strain lacks cell wall alpha-1,3-glucan |
-, 759245 |
