EC Number   |
Protein Variants |
Reference |
|---|
    2.4.1.175 | D241K |
inactive using UDP-N-acetyl-beta-D-galactosamine as substrate |
-, 703920 |
| 2.4.1.175 | D367A |
site-directed mutagenesis, a soluble mutant is generated by replacing the first 36 amino acids of ChGn-2 D367A with a cleavable insulin signal sequence and the protein A IgG-binding domain, inactive mutant |
721529 |
| 2.4.1.175 | D521K |
inactive using UDP-N-glucuronic acid as substrate |
-, 703920 |
| 2.4.1.175 | F362S |
naturally occuring missense mutation of the CHSY1 activity-related, highly conserved residue, the mutant protein has a 56% decrease in GalNAcT-II activity and a 55% decrease in GlcAT-II activity compared to the wild-type |
735696 |
| 2.4.1.175 | more |
both CHSY1 expression and CS56 staining are dramatically decreased in CHSY1-silenced cells |
759875 |
| 2.4.1.175 | more |
deletion of the ChGn-2 gene significantly reduces LDL retention in the DIT mouse model. Chondroitin sulfate N-acetylgalactosaminyltransferase-2 deletion alleviates lipoprotein retention in early atherosclerosis and attenuates aortic smooth muscle cell migration. A functional assay of ASMCs prepared from ChGn-2-/- mice displays abrogation of platelet-derived growth factor (PDGF)-mediated migration via reduced PDGF receptor phosphorylation. Evaluation of LDL retention in a diffuse intimal thickening (DIT) model using partial carotid ligation on ChGn-2/LDL receptor double knockout (ChGn-2-/- / LDLr-/-) mice |
-, 758734 |
| 2.4.1.175 | more |
gene silencing via shRNA sequences targeting Rattus norvegicus Smad2, Smad3, CHSY1, c-jun and Sp1, the shRNAs are transfected using a lentiviral vector |
735915 |
| 2.4.1.175 | more |
generation of chondroitin sulfate (CS) N-acetylgalactosaminyltransferase-1 (T1) gene knockout (KO) mice, Ehlers-Danlos syndrome (EDS)-like phenotype and phenotypic features, histological analysis, detailed overview. The protein levels of collagen type 1 and Wnt3a are significantly decreased by T1 gene knockdown by T1KD. Downregulation of collagen type 1 and Wnt3a by T1 gene knockdown in vitro and in vivo |
760165 |
| 2.4.1.175 | more |
generation of CSS2-/- mice, phenotype, overview |
723586 |
| 2.4.1.175 | more |
silencing of ChGn-2 in L cells by shRNA |
721529 |
