   2.4.1.140 | more |
generation of a truncated version of alternansucrase, mutant ASRDELTA2 (ASR (Met1-Ala2057)), three-dimensional structure determination and analysis. Unusual repeated sequences named APY repeats are identified at the C-terminal end of the protein. Their deletion in the mutant ASR C-APY-del (Met1-Gly1425) does not impact the product profile but significantly increases the enzyme half-life time at 50°C by 5fold compared to the full-length ASR. Generation of several truncated forms: ASRDELTA2, ASRDELTA3, ASRDELTA4, and ASRDELTA5, which are deleted, respectively, of the signal peptide (Met1-His38), one predicted disordered region (Met1-Asp78), two predicted disordered regions (Met1-Pro152), and the entire domain V (Met1-Ser398 and Gln1305-Gly1425). Domain organization of ASR truncated mutants, catalytic sites and domain properties of wild-type and mutant enzymes, detailed overview |
-, 758556 |
