EC Number |
Protein Variants |
Reference |
---|
2.4.1.135 | C301A |
mutant is not N-glycosylated, molecular weight is about 4000 Da less than that of the wild-type protein, enzyme is completely inactive |
393088 |
2.4.1.135 | C33A |
mutation abolishes the ability of the protein to form dimers |
393088 |
2.4.1.135 | D113A |
inactive mutant enzyme. Mutant enzyme is produced in a slightly lower amount compared with the wild-type enzyme |
677013 |
2.4.1.135 | D113E |
KM-value for UDP-glucuronate is 3.2fold higher than wild-type value |
677013 |
2.4.1.135 | D113N |
KM-value for UDP-glucuronate is 12.7fold higher than wild-type value |
677013 |
2.4.1.135 | D194A |
about 85% of wild-type activity |
659240 |
2.4.1.135 | D194A/D195A |
inactive mutant protein |
659240 |
2.4.1.135 | D194A/D196A |
inactive mutant protein |
659240 |
2.4.1.135 | D194E |
about 85% of wild-type activity |
659240 |
2.4.1.135 | D195A |
about 25% of wild-type activity |
659240 |