EC Number   |
Protein Variants |
Reference |
|---|
   2.4.1.120 | more |
construction of a stable seed-specific suppressed transgenic Brassica napus strain with highly reduced enzyme activity by dsRNAi-based gene silencing, utilization of the Agrobacterium tumefaciens infection system, analysis of sinapate ester pattern, 80% reduction of sinapate ester content, 72% reduced sinapine content |
660105 |
| 2.4.1.120 | more |
construction of UGT84A9-suppressing (UGT84A9i) lines, suppression of UGT84A9 under control of the seed-specific NAPINC promoter is maintained in cotyledons during the first two weeks of seedling development. Compared to the wild-type, the UGT84A9i lines display a dramatically altered metabolic phenotype during late seed and early seedling development, in both UGT84A9i lines, the level of sinapine is reduced to about 50% of the wild type level, overview. Determination of metabolite levels in four developmental stages of wild-type and mutants subjected to hierarchical cluster analysis reveal seven metabolite groups |
736958 |
| 2.4.1.120 | more |
two genes are isolated from Brassica juncea and eight silencing constructs are developed using three different RNA silencing approaches viz. antisense RNA, RNAi and artificial microRNA. Transgenics in Brassica juncea are developed following Agrobacterium-mediated transformation. From a total of 1232 independent T0 transgenic events obtained using eight silencing constructs, 25 homozygous lines showing single gene inheritance are identified in the T2 generation. Reduction of seed sinapine content in these lines range from 15.8% to 67.2% compared to wild-type. Development of suppression constructs for SGT, development of silencing constructs for SGT genes viz. antisense RNA (BjSGTAS for SGT), RNAi (BjSGTRNAi for SGT), and artificial microRNA (BjSGTamiR38 and BjSGTamiR40 for SGT), genetic analysis of T2 transgenics and establishment of homozygous lines |
760028 |
