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EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.102C100S mutant enzyme with 10% of wild-type activity 658191
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.102C151S inactive mutant protein 658191
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.102C172S inactive mutant protein 658191
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.102C199S inactive mutant protein 658191
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.102C217S Cys217 although not required for activity per se, represents the only thiol that causes enzyme inactivation when modified. KM-value for UDP-N-acetyl-D-glucosamine is about 5% higher than the wild-type value, the Km-value for Galbeta(1-3)GalNAcalpha-p-nitrophenol is about 20% higher than the wild-type value.Unlike the wild-type enzyme the mutant enzyme can be stored at 4°C for at least 3 months without any loss of activity. The mutant enzyme is insensitive to DTNB, iodoacetamide, NEM, HgCl2 and ZnCl2 659276
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.102C217S mutant enzyme with 41% of wild-type activity. Similar Km and Vmax values for Galbeta(1-3)GalNAcalpha-p-nitrophenol, Km-value for UDP-N-acetyl-D-glucosamine is 2.9fold higher than wild-type value. In contrast to wild-type enzyme, the mutant enzyme is not inhibited by sulfhydryl reagents and is not stimulated by reducing agents 658191
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.102C217S the mutant is catalytically active 722983
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.102C372S inactive mutant protein 658191
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.102C381S inactive mutant protein 658191
Display the word mapDisplay the reaction diagram Show all sequences 2.4.1.102C413S inactive mutant protein 658191
Results 1 - 10 of 29 > >>
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