EC Number   |
Protein Variants |
Reference |
|---|
   2.3.2.12 | A2451U |
the base identity at this highly conserved residue is not absolutely critical for peptide bond synthesis |
673179 |
| 2.3.2.12 | A2451U |
with puromycin as an acceptor substrate, the catalytic rate of the mutant enzyme decreases about 150fold relative to wild type enzyme |
673179 |
| 2.3.2.12 | C74G |
1.9fold inhibition in the rate of translocation of tRNA |
675409 |
| 2.3.2.12 | G2252C |
the rate of peptidyl transferase reaction is 100fold decreased |
675409 |
| 2.3.2.12 | K372A |
the mutant shows 7% of wild type activity |
736833 |
| 2.3.2.12 | K372A/R437A |
the mutant shows 2% of wild type activity |
736833 |
| 2.3.2.12 | more |
changes of 23S rRNA nucleotides in the 2585 region of the peptidyl transferase center, G2583A and U2584C, reduce maximum induction of tna operon expression by tryptophan in vivo without affecting the concentration of tryptophan necessary to obtain 50% induction. The growth rate of strains with ribosomes with either of these changes is not altered appreciably. In vitro analyses show that tryptophan is not as efficient in protecting TnaC-tRNAPro from puromycin action as wild-type ribosomes. However, added tryptophan does prevent sparsomycin action as it normally does with wild-type ribosomes. These two mutational changes act by reducing the ability of ribosome-bound tryptophan to inhibit peptidyl transferase activity rather than by reducing the ability of the ribosome to bind tryptophan |
698614 |
| 2.3.2.12 | more |
introduction of individual 23S rRNA mutations associated with pleuromutilin resistance at positions 2055, 2447, 2504 and 2572, Escherichia coli numbering, into a Mycobacterium smegmatis strain with a single rRNA operon. The single mutations each confer a significant and similar degree of valnemulin resistance and those at 2447 and 2504 also confer cross-resistance to other antibiotics that bind to the peptidyl transfer center. The introduced mutations cause structural perturbations at the peptidyl transfer center and reduce binding of pleuromutilin antibiotics |
700279 |
| 2.3.2.12 | more |
lack of pseudouridine at position 2504 of 23S rRNA significantly increases the susceptibility of Escherichia coli to peptidyl transferase inhibitors |
699543 |
| 2.3.2.12 | more |
mutant DELTAldtC is hypersusceptible to imipenem |
-, 736769 |
