EC Number   |
Protein Variants |
Reference |
|---|
    2.3.1.46 | A195T |
site-directed mutagenesis |
735854 |
| 2.3.1.46 | A200E |
site-directed mutagenesis |
735854 |
| 2.3.1.46 | A28V |
the mutation leads to 2.94fold improved L-methionine productivity in Escherichia coli DELTAIJA strain |
757283 |
| 2.3.1.46 | C142A |
site-directed mutagenesis, the mutant is inactive |
671625, 672232 |
| 2.3.1.46 | C142S |
site-directed mutagenesis, the mutant is almost inactive |
671625 |
| 2.3.1.46 | C142S |
site-directed mutagenesis, the mutant is inactive |
672232 |
| 2.3.1.46 | C90A |
site-directed mutagenesis, the mutant shows only slightly reduced activity compared to the wild-type enzyme |
672232 |
| 2.3.1.46 | C90S |
site-directed mutagenesis, the mutant shows only slightly reduced activity compared to the wild-type enzyme |
672232 |
| 2.3.1.46 | D218G |
site-directed mutagenesis |
735854 |
| 2.3.1.46 | E213V |
15-20% faster growth at 36-41°C, lower growth rate at 44°C (64% of control), no growth at 45°C |
701784 |
