EC Number |
Protein Variants |
Reference |
---|
2.3.1.4 | biotechnology |
engineering of a metabolic pathway for high level production of N-acetylglucosamine in Escherichia coli by overexpressing Escherichia coli glucosamine synthase (GlmS) and Saccharomyces cerevisiae glucosamine-6-phosphate acetyltransferase (GNA1) |
673392 |
2.3.1.4 | C157A |
mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity |
755991 |
2.3.1.4 | E156A |
Km: 1.2 mM (substrate: glucosamine 6-phosphate), kcat = 9.7/sec (substrate: glucosamine 6-phosphate) |
686767 |
2.3.1.4 | E156D |
Km: 0.175 mM (substrate: glucosamine 6-phosphate), kcat = 19.3/sec (substrate: glucosamine 6-phosphate) |
686767 |
2.3.1.4 | E185Y |
Km (mM): 0.03 (D-glucosamine 6-phosphate), 0.04 (acetyl-CoA), kcat (1/sec): 1.34 (D-glucosamine 6-phosphate) |
685012 |
2.3.1.4 | G183E |
Km (mM): 0.056 (D-glucosamine 6-phosphate), 0.1 (acetyl-CoA), kcat (1/sec): 0.34 (D-glucosamine 6-phosphate) |
685012 |
2.3.1.4 | G68S |
the mutant called lignescens, a temperature-sensitive mutant that exhibits ectopic lignin deposition and growth defects under high-temperature conditions, is due to single base transition G68S in glucosamine-6-phosphate N-acetyltransferase. When exposed to the restrictive temperature, the mutant strain contains a significantly smaller amount of UDP-GlcNAc than the wild type. The growth defects and ectopic lignification of the mutant are suppressed by the addition of UDP-GlcNAc. N-glycans are reduced and luminal binding protein 3, a typical UPR gene, is expressed in the mutant strain at the restrictive temperature. Treatment with UPR-inducing reagents phenocopies the mutant |
720693 |
2.3.1.4 | L138A |
mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity. Ratio of butanoyl-to-acetyltransferase activity is increased to 1 |
755991 |
2.3.1.4 | L155A |
mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity |
755991 |
2.3.1.4 | L155A/N161A |
mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity |
755991 |