EC Number   |
Protein Variants |
Reference |
|---|
    2.3.1.23 | C211F |
C211 is essential for activity |
705001 |
| 2.3.1.23 | C211R |
C211 is essential for activity |
705001 |
| 2.3.1.23 | C211S |
C211 is essential for activity |
705001 |
| 2.3.1.23 | C47S |
site-directed mutagenesis, construction of mutant endothelial cells via lentivirus transfection. The C47S mutant protein does not express peroxidase activity, but both PLA2 and LPCAT activities are preserved |
-, 757372 |
| 2.3.1.23 | D140A |
site-directed mutagenesis, construction of mutant endothelial cells via lentivirus transfection. The D140A mutant protein retains full peroxidase activity |
-, 757372 |
| 2.3.1.23 | D31A |
site-directed mutagenesis, construction of mutant endothelial cells via lentivirus transfection |
757372 |
| 2.3.1.23 | D31A |
site-directed mutagenesis, construction of mutant endothelial cells via lentivirus transfection, the mutant loses almost all LPCAT activity, but retains PLA2 activity |
-, 757372 |
| 2.3.1.23 | H129A |
site-directed mutagenesis, mutation of a residue in AGPAT motif I (HxxxxD), the mutant shows no LPEAT activity |
755989 |
| 2.3.1.23 | H26A |
site-directed mutagenesis, breeding of H26A Prdx6 knock-in mutant mice, the final targeting construct is linearized, sequence verified, and electroporated into C57Bl/6J ES cells (EAP6 ES cells) for insertion of the mutant sequences into the mouse genome by homologous recombination, positive clones are used for blastocyst injection into CD-1/BALB/c mice, chimeric H26A Prdx6 mice are bred to C57Bl/6J wild-type mice and the resulting heterozygotic mice are bred to homozygosity. The H26A mutant retains the ability to reduce short chain hydroperoxides, but cannot reduce phospholipid hydroperoxides, as they do not bind to the phospholipid substrate |
-, 757372 |
| 2.3.1.23 | H26A |
site-directed mutagenesis, construction of mutant endothelial cells via lentivirus transfection |
757372 |
