EC Number |
Protein Variants |
Reference |
---|
2.3.1.212 | C197G |
site-directed mutagenesis, the mutant shows an unaltered product pattern compared to the wild-type enzyme |
721796 |
2.3.1.212 | C197T |
site-directed mutagenesis, the mutant shows an unaltered product pattern compared to the wild-type enzyme |
721796 |
2.3.1.212 | G256L |
site-directed mutagenesis, the mutant shows 50% reduced activity but an unaltered product pattern compared to the wild-type enzyme |
721796 |
2.3.1.212 | I214L/L215F |
site-directed mutagenesis, the mutant restores chalcone-forming activity |
721796 |
2.3.1.212 | I214L/L215F |
site-directed mutagenesis, the mutation restores the active site residues of chalcone synthase, the mutant shows chalcone-forming activity, EC 2.3.1.74. The mutant enzyme thus exhibits 36fold decreases in kcat/Km for 4-coumaroyl-CoA and 20fold decreases in kcat/Km for malonyl-CoA compared with wild-type BAS, kinetics of chalcone naringenin-forming activity at pH 6.5, overview |
722633 |
2.3.1.212 | L132A |
site-directed mutagenesis, the substitution expands the product chain length to produce 4-coumaroyltriacetic acid lactone after three condensations with malonyl-CoA, but without the formation of the aromatic ring system |
721798 |
2.3.1.212 | L132C |
site-directed mutagenesis, the substitution expands the product chain length to produce 4-coumaroyltriacetic acid lactone after three condensations with malonyl-CoA, but without the formation of the aromatic ring system |
721798 |
2.3.1.212 | L132F |
site-directed mutagenesis, replacement of Leu132 with bulky aromatic residues, Phe, Tyr and Trp, causes a 1.2fold increase in the benzalacetone-forming activity at pH 8.0, whereas the bisnoryangonin-forming activity is retained or significantly decreased at pH 6.5 |
721798 |
2.3.1.212 | L132G |
site-directed mutagenesis, no altered activity compared to the wild-type enzyme |
721798 |
2.3.1.212 | L132P |
site-directed mutagenesis, the L132P mutant exhibits drastically decreased benzalacetone- and bisnoryangonin-forming activities |
721798 |