EC Number   |
Protein Variants |
Reference |
|---|
   2.3.1.198 | G163R |
isolation of a set of fruit brightness mutants (named glossy mutants) with abnormal fruit cutin amounts/compositions from the EMS-mutagenized tomato mutant population (cv Micro-Tom). The glossy mutant line P23F12 exhibits enhanced fruit brightness and a reduced cutin load. Whole-genome sequencing and gene mapping show that a single G613A nucleotide transition in the first exon leading to a G163R nonsynonymous mutation in gene SlGPAT (Solyc09g014350) is responsible for the mutant glossy fruit phenotype, i.e. mutant gpat6-a, overview. The inactive mutant gpat6-a has a fruit cuticle that shows reduced levels of C16 cutin monomers. No differences are observed in total wax load between wild-type and gpat6-a fruits, but differences in wax composition are apparent, such as increased or decreased levels of several minor C25 to C30 alkanes in the gpat6-a mutant. Since both even- and odd-numbered alkanes display similar trends, these variations are likely independent of the alkane biosynthetic pathways. The most striking wax compositional change in the gpat6-a mutant is an approximately 5fold increase in delta- and alpha-amyrin levels and to a lesser extent in beta-amyrin levels. In contrast to waxes, the total cutin load in gpat6-a is reduced by almost 3fold. MapMan-based general overview of genes differentially expressed between wild-type and gpat6-a mutant fruit |
758037 |
| 2.3.1.198 | more |
gene GPAT6 knockout phenotype, overview |
757823 |
| 2.3.1.198 | more |
generation of a T-DNA insertion mutant strain of GPAT6, determination of chlorophyll content and fatty acids composition of phosphatidylglycerol (PG) in Arabidopsis thaliana GPAT6 mutant line, overview. In the seedling stage, chlorophyll content, the photochemical efficiency of PSII, PSI oxidoreductive activity (1I/Io), and the unsaturated fatty acid content of PG decrease less in overexpressing strains and more in mutant strains than that in wild-type under salt stress. Overexpression of glycerol-3-phosphate acyltransferase from Suaeda salsa improves salt tolerance in Arabidopsis thaliana deficient in GPAT6. The overexpression of SsGPAT alleviates the photoinhibition of PSII and PSI under salt stress by improving the unsaturated fatty acid content of PG |
-, 756821 |
| 2.3.1.198 | more |
glycerol content is strongly reduced in both stem and leaf cutin from all Arabidopsis thaliana mutants analyzed (gpat4/gpat8). Knocking out of these genes individually or as a pair (gpat4/gpat8) leads to large reductions in Arabidopsis thaliana cutin monomer loads, especially in the major monomer octadeca-6,9-diene-1,18-dioate (C18:2 DCA) |
-, 757936 |
| 2.3.1.198 | more |
leaves of 6-wk-old tomato cv. Micro-Tom or gpat6-a mutant plants are subjected to a detached leaf infection assay, gpat6-a tomato fruits have increased cuticle permeability, gpat6-a tomato leaves have fewer stomata and show an increased rate of water loss compared with the wild-type. Mutant gpat6-a leaf epidermal cells possess a thicker cell wall. The gpat6-a leaf transcriptome reflects changes in cuticle and cell wall processes and stomatal patterning. Phenotype, overview |
757823 |
| 2.3.1.198 | more |
targeted disruption of PpGPAT2, DELTAppgpat2 phenotype, overview |
757825 |
| 2.3.1.198 | more |
targeted mutagenesis of PpGPAT4, DELTAppgpat4 phenotype, overview |
757825 |
