EC Number   |
Protein Variants |
Reference |
|---|
   2.1.1.57 | C178S |
point mutations, glutathione-S-transferase-tagged, exchange shows no effect |
639487 |
| 2.1.1.57 | C272S |
point mutations, glutathione-S-transferase-tagged, exchange shows no effect |
639487 |
| 2.1.1.57 | C272S/K175C/R209K |
2-bromoethylamine reveals 64% target site modification in a overnight reaction at 37°C, unmodified mutant has no detectable methyltransferase activity, whereas the modified protein is active, exhibiting 20-30% of the specific catalytic rate of wild-type |
672205 |
| 2.1.1.57 | D106A |
the mutant shows 38% of wild type activity |
720878 |
| 2.1.1.57 | D113A |
about 110% substrate binding and activity compared to the wild type enzyme |
704920 |
| 2.1.1.57 | D129A |
about 40% substrate binding and activity compared to the wild type enzyme |
704920 |
| 2.1.1.57 | D130A |
the mutant shows 2% of wild type activity |
720878 |
| 2.1.1.57 | D141A |
RNA synthesis is severely impaired in this mutant |
758536 |
| 2.1.1.57 | D1762A |
with pinpoint plaque morphologies and replication defects in single-step growth assays, viral RNA and protein synthesis is diminished |
675702 |
| 2.1.1.57 | D182A |
m7G binding pocket mutant, mutagenesis, C-terminal truncated by 26 amino acids, lacks specific m7G-contact side chains and shows reduced activity, but remains cap-dependent |
639484 |
