EC Number   |
Protein Variants |
Reference |
|---|
   2.1.1.240 | D174A |
site-directed mutagenesis, almost inactive mutant |
735437 |
| 2.1.1.240 | F167A |
site-directed mutagenesis, inactive mutant |
735437 |
| 2.1.1.240 | H261A |
site-directed mutagenesis, inactive mutant |
735437 |
| 2.1.1.240 | more |
construction of a biological platform to produce pterostilbene with the ROMT gene of Arabidopsis thaliana. Pterostilbene can be synthesized from intracellular L-tyrosine, which requires the activities of four enzymes: tyrosine ammonia lyase (TAL) from Saccharothrix espanaensis, p-coumarate:CoA ligase (CCL) from Nicotiana tabacum, stilbene synthase (STS) from Vitis vinifera, and resveratrol O-methyltransferase (ROMT). For the efficient production of pterostilbene in Escherichia coli, an engineered Escherichia coli strain P1 is used to increase the intracellular pool of L-tyrosine, which is the initial precursor of pterostilbene, with L-mehionine containing media to increase the intracellular pool of S-adenosyl-L-methionine (SAM). Pterostilbene production as high as 33.6 mg/l is achieved, which is about 3.6fold higher compared with that in the parental Escherichia coli strain harboring a plasmid for pterostilbene biosynthesis. Method, overview |
757597 |
| 2.1.1.240 | more |
development of an Escherichia coli system containing an artificial biosynthetic pathway that produces methylated resveratrol analogues, such as pinostilbene (3,4'-dihydroxy-5-methoxystilbene), 3,5-dihydroxy-4'-methoxystilbene, 3,4'-dimethoxy-5-hydroxystilbene, and 3,5,4'-trimethoxystilbene, from simple carbon sources. The artificial biosynthetic pathways contain a series of codon-optimized O-methyltransferase genes from sorghum in addition to the resveratrol biosynthetic genes |
735842 |
| 2.1.1.240 | more |
enhanced production of resveratrol derivatives in Nicotiana tabacum plants by improving the metabolic flux of intermediates in the phenylpropanoid pathway. Generation of transgenic Nicotiana tabacum SR1 plants (STS-OX and ROST-OX) expressing the RpSTS gene encoding stilbene synthase from rhubarb (Rheum palmatum cv. Jangyeop) and the RpSTS and VrROMT genes encoding resveratrol O-methyltransferase from frost grape (Vitis riparia) under the control of 35S promoter. Phenotypes and method development and evaluation, overview |
758021 |
| 2.1.1.240 | more |
functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum and in Vitis vinifera cell culture for production of pterostilbene, kaempferol, and quercetin |
742957 |
| 2.1.1.240 | more |
metabolic engineering of stilbene biosynthesis, production of resveratrol and its monomethylated derivative pinostilbene as the major product from 4-coumaric acid in Escherichia coli through coexpression of multiple enzymes (cinnamate/4-coumarate:coenzyme A ligase, stilbene synthase, resveratrol O-methyltransferase) responsible for stilbene biosynthesis. When 4-coumaric acid is fed as the precursor, maximum levels of resveratrol and pinostilbene are produced by recombinant Escherichia coli cells co-expressing Streptomyces coelicolor cinnamate/4-coumarate:coenzyme A ligase ScCCL and Rheum palmatum stilbene synthase RpSTSsyn, or Streptomyces coelicolor ScCCL, RpSTSsyn and Sorghum bicolor resveratrol O-methyltransferase SbROMT3syn, respectively. Method evaluation and optimization |
736807 |
| 2.1.1.240 | W258A |
site-directed mutagenesis, inactive mutant |
735437 |
