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EC Number Protein Variants Commentary Reference
Display the reaction diagram Show all sequences 2.1.1.207K42E site-directed mutagenesis, inactive mutant -, 736900
Display the reaction diagram Show all sequences 2.1.1.207K81E site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme -, 736900
Display the reaction diagram Show all sequences 2.1.1.207more construction of gene trmL deletion mutants -, 736900
Display the reaction diagram Show all sequences 2.1.1.207more generation of trmL+ and trmL- PBAD-rpoS990-lacZ translational fusions (in rssB- backgrounds) cells, executed for PBAD-rpoS990-lacZ translational fusions with rpoS UUA-Leu CUX-Leu mutations (leu*1)-strains KMT36002 and KMT36010, for rpoS UUG-Leu to CUX-leu mutation (leu*2)-strains KMT37002 and KMT37010, and for rpoS with both UUA-Leu to CUX-Leu and UUG-Leu to CUX-Leu mutations (leu*3)-strains KMT33001 and KMT33013, overview. Comparison of expression of PBAD-rpoS990-lacZ translational fusions carrying wild-type UUX leucine codons with derivatives in which UUX codons are changed to CUX codons, in the presence and absence of TrmL or TusA. The absence of these proteins, and therefore presumably the modifications they catalyze, both abolish PBAD-rpoS990-lacZ translation activity. UUX-Leu to CUX-Leu codon mutations in rpoS suppress the trmL requirement for PBAD-rpoS990-lacZ expression 756162
Display the reaction diagram Show all sequences 2.1.1.207R104E site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme 736900
Display the reaction diagram Show all sequences 2.1.1.207R129E site-directed mutagenesis, inactive mutant 736900
Display the reaction diagram Show all sequences 2.1.1.207R20E site-directed mutagenesis, inactive mutant 736900
Display the reaction diagram Show all sequences 2.1.1.207R28E site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type enzyme 736900
Display the reaction diagram Show all sequences 2.1.1.207R43E site-directed mutagenesis, inactive mutant 736900
Display the reaction diagram Show all sequences 2.1.1.207R45E site-directed mutagenesis, inactive mutant 736900
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