EC Number   |
Protein Variants |
Reference |
|---|
   2.1.1.142 | F82I |
continues to catalyze strongly both the first and second C1-transfer activities and generates product sets similar to the control |
671596 |
| 2.1.1.142 | F82L |
first C1-transfer activity is relatively unaffected, loss of the second C1-transfer activity |
671596 |
| 2.1.1.142 | F85L |
first C1-transfer activity is relatively unaffected, loss of the second C1-transfer activity |
671596 |
| 2.1.1.142 | F91L |
first C1-transfer activity reduced from the control activity, loss of the second C1-transfer activity |
671596 |
| 2.1.1.142 | F93L |
first C1-transfer activity reduced from the control activity, loss of the second C1-transfer activity |
671596 |
| 2.1.1.142 | more |
construction of SMT1-deficient mutants with altered phenotype, reduced fertility and root growth defects, transgenic plant seedlings are sensitive against brassinosteroids and transfection with a genomic clone or a SMT1 cDNA can complement the mutants |
485338 |
| 2.1.1.142 | more |
GhSMT2-1 and GhSMT2-2 have high homology with the SMT2 from Arabidopsis thaliana and Nicotiana tabacum. The typical conserved structures characterized by the sterol C-24 methyltransferase, such as region I (LDVGCGVGGPMRAI), region II (IEATCHAP), and region III (YEWGWGQSFHF), are present in both deduced proteins |
688128 |
| 2.1.1.142 | more |
overexpression of SMT2 gene in a mutant strain leads to an accumulation of sitosterol and to an altered phenotype, some of the changes can be restored by brassinosteroid treatment, some cannot, sterol profile of wild-type and mutant plants |
349712 |
| 2.1.1.142 | W87L |
first and second C1-transfer activities are greatly reduced from the control activities |
671596 |
| 2.1.1.142 | Y81F |
site-directed mutagenesis, the mutation causes a preferential change in affinity for DELTA24-sterols that affords alter partitioning in the direction of 24-ethyl(idene) product formation |
-, 718966 |
