EC Number |
Protein Variants |
Reference |
---|
2.1.1.11 | C111S |
0.1% of wild-type activity |
757980 |
2.1.1.11 | C111S/C115S |
only residual in vitro activity under reducing conditions, almost complete loss of activity under non-reducing conditions |
757980 |
2.1.1.11 | C115S |
20% of wild-type activity, strong stimulation by dithiothreitol |
757980 |
2.1.1.11 | C115S/C177S |
only residual in vitro activity under reducing conditions, almost complete loss of activity under non-reducing conditions |
757980 |
2.1.1.11 | C177S |
residue is responsible for redox-dependent enzyme activation. In absence of dithiothreitol, increased activity compared with wild-type. Only slight activation by dithiothreitol |
757980 |
2.1.1.11 | H139A |
loss of more than 80% of methyltransferase activity, mutant has comparable Kd values for Mg-phosphate as the wild type protein |
734272 |
2.1.1.11 | H139N |
loss of more than 80% of methyltransferase activity, mutant has comparable Kd values for Mg-phosphate as the wild type protein |
734272 |
2.1.1.11 | H139Q |
loss of more than 80% of methyltransferase activity, mutant has comparable Kd values for Mg-phosphate as the wild type protein |
734272 |
2.1.1.11 | L225F |
mutation yellow-green leaf 18, leads to yellow-green leaves, decreased chlorophyll level, and climate-dependent growth differences |
756823 |
2.1.1.11 | L271P |
naturally occuring mutation, Mg-protoporphyrin IX accumulated in the mutant, a low activity of the mutant protein is not excluded |
720712 |