EC Number   |
Protein Variants |
Reference |
|---|
   1.8.4.2 | C101A |
site-directed mutagenesis, the mutation of the cysteine at position 101 to alanine results in a high-molecular-weight complex that is positive for MdbA and VKOR by immunoblotting and is absent in other alanine substitution mutants and the C93A/C101A double mutation and after treatment with the reducing agent 2-mercaptoethanol |
745224 |
| 1.8.4.2 | C29S |
specific activity about 5% of wild-type |
762613 |
| 1.8.4.2 | C46A |
site-directed mutagenesis |
765010 |
| 1.8.4.2 | C49A |
site-directed mutagenesis, SdbAC49A forms a mixed disulfide with SpeAC87A |
765010 |
| 1.8.4.2 | C57S |
site-directed mutagenesis, reduced activity |
394863 |
| 1.8.4.2 | C57S/C60S |
site-directed mutagenesis, no activity |
394863 |
| 1.8.4.2 | C58S |
complete loss of oxidoreductase activtiy |
741551 |
| 1.8.4.2 | C60S |
site-directed mutagenesis, no activity |
394863 |
| 1.8.4.2 | C87S |
specific activity simiular to wild-type |
762613 |
| 1.8.4.2 | C89A |
site-directed mutagenesis, the single-cysteine active site variant, SdbAC89A, forms a number of mixed disulfide complexes in the mutant |
-, 765006 |
