EC Number   |
Protein Variants |
Reference |
|---|
   1.7.2.2 | H264N |
mutant is unable to catalyze nitrite reduction but able to reduce hydroxylamine. The mutant simultaneously binds nitrite and electrons at the catalytic heme |
742746 |
| 1.7.2.2 | H268M |
one of the EPR-silent heme's histidine axial ligands is replaced with a methionine |
741912 |
| 1.7.2.2 | H277N |
mutant is unable to catalyze nitrite reduction but able to reduce hydroxylamine. The mutant simultaneously binds nitrite and electrons at the catalytic heme |
-, 742746 |
| 1.7.2.2 | K100H |
mutagenesis of the hem-binding motif CWSCK results in almost complete loss of formate-dependent nitrite reduction |
288633 |
| 1.7.2.2 | K100I |
mutagenesis of the hem-binding motif CWSCK results in almost complete loss of formate-dependent nitrite reduction |
288633 |
| 1.7.2.2 | K100L |
mutagenesis of the hem-binding motif CWSCK results in almost complete loss of formate-dependent nitrite reduction |
288633 |
| 1.7.2.2 | K119H |
site-directed mutagensis, mutation of the essential lysine residue of the non-canonical HBM (CX2CK), inactive mutant. The mutant NrfA protein displays a similar pattern of rapid degradation like the wild-type without maturation |
743309 |
| 1.7.2.2 | K119L |
site-directed mutagensis, mutation of the essential lysine residue of the non-canonical HBM (CX2CK), inactive mutant. The mutant NrfA protein displays a similar pattern of rapid degradation like the wild-type without maturation |
743309 |
| 1.7.2.2 | more |
anaerobically grown Escherichia coli nrf mutants are more sensitive to NO radicals than the parent strain |
698876 |
| 1.7.2.2 | more |
costruction of a DELTAnrfA mutant enzyme knockout strain, expression of the wild-type enzyme from vector pHG101 can restore the enzyme activity, but not expression of enzyme mutants K119L and K119H |
743309 |
