EC Number   |
Protein Variants |
Reference |
|---|
    1.5.3.13 | H69E |
compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value |
742614 |
| 1.5.3.13 | H69Q |
compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value |
742614 |
| 1.5.3.13 | K315M |
compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value |
742614 |
| 1.5.3.13 | K315M |
mutation has no effect on the kcat/Kamine profile for spermine. The kred value with N1-acetylspermine is 1.8fold lower in the mutant protein, and the pKa in the k(red)-pH profile with N1-acetylspermine shifts to 7.8. K315 does not play a critical role in amine oxidation by PAO |
691010 |
| 1.5.3.13 | K315M |
site-directed mutagenesis, the mutant shows altered kinetics compared to the wild-type enzyme |
691010 |
| 1.5.3.13 | N313A |
dramatic reduction in catalytic efficiency with substrate N1-acetylspermine |
764138 |
| 1.5.3.13 | N313D |
dramatic reduction in catalytic efficiency with substrate N1-acetylspermine |
764138 |
| 1.5.3.13 | N313L |
dramatic reduction in catalytic efficiency with substrate N1-acetylspermine |
764138 |
| 1.5.3.13 | N313T |
dramatic reduction in catalytic efficiency with substrate N1-acetylspermine |
764138 |
| 1.5.3.13 | T467S |
compared with the wild-type enzyme the mutant enzyme shows lower Vmax value and higher Km-value |
742614 |
