EC Number   |
Protein Variants |
Reference |
|---|
   1.3.99.29 | E143K |
naturally occcuring mutation, mutation pds1-1 |
-, 726303 |
| 1.3.99.29 | L505F |
site-directed mutagenesis, the mutation enhances enzyme desaturation activity by 29% compared to the wild-type enzyme, L505F exhibits a 27.7fold higher resistance to the herbicide norflurazon |
-, 726419 |
| 1.3.99.29 | L516F |
naturally occuring mutant strain E17, the mutant enzyme is insensitive, with 31fold higher resistane compared to the wild-tpe enzyme, to inhibition by norflurazon.The mutant also shows higher catalytic efficiency than the wild-type enzyme. Induced by high-light irradiation or glucose, E17 produces 44 or 36% more astaxanthin than the wild-type when cultured in media without norflurazon |
-, 726248 |
| 1.3.99.29 | L516R |
the mutant show reduced enzyme activity compared to the wild-type enzyme |
-, 726248 |
| 1.3.99.29 | L64F/E143K |
site-directed mutagenesis, mutation pds1-5, with in-frame deletion of PSY residues 7-14 |
-, 726303 |
| 1.3.99.29 | L64P |
site-directed mutagenesis, mutation pds1-4, with in-frame deletion of PSY residues 7-14 |
-, 726303 |
| 1.3.99.29 | L64P/E143K |
site-directed mutagenesis, mutation pds1-2, with in-frame deletion of PSY residues 7-14 |
-, 726303 |
| 1.3.99.29 | L64P/K90M/E143K |
site-directed mutagenesis, mutation pds1-6, with in-frame deletion of PSY residues 7-14 |
726303 |
| 1.3.99.29 | more |
possibility of engineering the carotenogenic pathway for improved carotenoid production in microalgae, overview |
-, 726419 |
| 1.3.99.29 | more |
the naturally occuring pds1-1 allele shows a mutant with reduced enzyme activity and growth defects, isolation of pds1-2 as an intragenic enhancer of pds1-1, the pds1-2 and pds1-3 allele products completely lack PDS activity, pds1-3 is a null allele derived from DNA insertional mutagenesis. The null mutants accumulate only phytoene and no other carotenoids. All three phytoene-accumulating mutants exhibit lower growth rates and reduced plating efficiency compared to wild-type cells and white phytoene synthase mutants. DNA insertional mutagenesis, phenotypes, detailed overview |
-, 726303 |
