EC Number   |
Protein Variants |
Reference |
|---|
  1.3.99.28 | F166I |
mutation changes the product of phytoene desaturation from neurosporene to lycopene |
712351 |
| 1.3.99.28 | H12Q |
mutation has little effect on the product formation |
712351 |
| 1.3.99.28 | L153P/L278P |
site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme |
711020 |
| 1.3.99.28 | L208F |
site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme |
711020 |
| 1.3.99.28 | L208P |
site-directed mutagenesis, the mutant shows altered secondary structure compared to the wild-type enzyme |
711020 |
| 1.3.99.28 | M402T |
mutation has a negative effect on percent lycopene production |
712351 |
| 1.3.99.28 | more |
competition between lycopene cyclase and the phytoene desaturases modified the distribution between carotene intermediates when expressed in yeast in the context of the full beta-carotene production pathway |
-, 759253 |
| 1.3.99.28 | more |
directed evolution is used to change the product of Rhodobacter sphaeroides phytoene desaturase (crtI gene product), a neurosporene-producing enzyme, to lycopene. Two generations of random mutagenesis are performed, from which three positive mutants are isolated and sequenced. Site-directed mutagenesis is used to determine the effect of each amino acid change |
712351 |
| 1.3.99.28 | more |
functional complementation of heterogeneous phytoene desaturases (CrtIs) from 5 carotenogenic microorganisms, overview |
-, 724038 |
| 1.3.99.28 | T256M/D355G/L424P |
site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme |
711020 |
