EC Number   |
Protein Variants |
Reference |
|---|
   1.3.7.6 | C71A |
site-directed mutagenesis, mutant shows reduced activity compared to wild-type |
763016 |
| 1.3.7.6 | D105E |
site-directed mutagenesis, the mutant catalyzes only the first reaction step, i.e. the formation of 15,16-dihydrobiliverdin, reaction of EC 1.3.7.2 |
724228 |
| 1.3.7.6 | D105E |
site-directed mutagenesis, the mutant fully retains the ability to catalyse the first reduction at the 15,16-methine bridge, but cannot catalyse the second reduction at the A-ring 2,3,31,32-diene system, thereby yielding 15,16-DHBV as the final product |
724220 |
| 1.3.7.6 | D105N |
site-directed mutagenesis, inactive mutant |
724228 |
| 1.3.7.6 | D206E |
site-directed mutagenesis, the mutant catalyzes only the second reaction step, i.e. the formation of (3Z)-phycoerythrobilin from 15,16-dihydrobiliverdin, reaction of EC 1.3.7.3 |
724228 |
| 1.3.7.6 | D206E |
site-directed mutagenesis, the mutant fully retains the ability to catalyse the first reduction at the 15,16-methine bridge, but cannot catalyse the second reduction at the A-ring 2,3,31,32-diene system, however, in this mutant the reaction can be pushed further to produce (3Z)-phycoerythrobilin by a 10fold increase in ferredoxin concentration |
724220 |
| 1.3.7.6 | D206N |
site-directed mutagenesis, the mutant catalyzes only the first reaction step, i.e. the formation of 15,16-dihydrobiliverdin, reaction of EC 1.3.7.2 |
724228 |
| 1.3.7.6 | D206N |
site-directed mutagenesis, the mutant fully retains the ability to catalyse the first reduction at the 15,16-methine bridge, but cannot catalyse the second reduction at the A-ring 2,3,31,32-diene system, thereby yielding 15,16-DHBV as the final product |
724220 |
| 1.3.7.6 | D55N |
site-directed mutagenesis |
763016 |
| 1.3.7.6 | D86N |
site-directed mutagenesis, inactive mutant |
763016 |
