EC Number   |
Protein Variants |
Reference |
|---|
   1.3.3.11 | H154S |
site-directed mutagenesis, active site mutant, even with substrate PQQ bound, the enzyme is still in the open conformation with helices alpha5b and alpha6 unfolded and the active site solvent accessible, no product formation |
-, 726458 |
| 1.3.3.11 | H84A |
active site mutant with reduced activity |
685167 |
| 1.3.3.11 | H84N |
active site mutant with reduced activity |
685167 |
| 1.3.3.11 | more |
construction of a pqqC gene disruption mutant JX22MT1 by the EZ-Tn5 transposon mutation, the mutant shows no antifungal activity against Fusarium oxysporum f. sp. lycopersici in contrast to the wild-type strain JX22. Antifungal activity at wild-type level is restored by introduction of the functional wild-type pqqC gene |
-, 744930 |
| 1.3.3.11 | more |
N60I mutation detected in PqqC deficiency mutant |
-, 657729 |
| 1.3.3.11 | R179S/Y175S |
site-directed mutagenesis, active site mutant, the mutant shows an open conformation with a reaction intermediate trapped in the active site, the intermediate is tricyclic but nonplanar, implying that it has not undergone oxidatio, R179S/Y175S shows acceptable substrate complex crystals |
-, 726458 |
| 1.3.3.11 | Y175F |
site-directed mutagenesis, active site mutant, the mutant shows a closed conformation indicating that Y175 is not required for the conformational change, no product formation |
-, 726458 |
