EC Number   |
Protein Variants |
Reference |
|---|
    1.3.1.84 | more |
construction of a gene SPO1914 disruption mutant by homologous recombination of suicide plasmids, the mutant is unable to grow on acrylate as the sole carbon source |
745800 |
| 1.3.1.84 | more |
construction of mutant DELTAacuI::kan. The introduction of codon-optimized genes SPO_1914 or yhdH into the DELTAacuI::kan mutant of Rhodobacter sphaeroides on plasmid pMA5-1 complements 3-hydroxypropionate-dependent growth of the mutant strain |
745213 |
| 1.3.1.84 | more |
metabolic engineering analysis of the the 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) cycle for improving the pathway flux, overview |
-, 745689 |
| 1.3.1.84 | more |
to construct an acrylic acid-producing pathway in Escherichia coli strain MG1655DELTArecADELTAendA(DE3)/pZL42, heterologous expression of malonyl-CoA reductase (MCR), malonate semialdehyde reductase (MSR), 3-hydroxypropionyl-CoA synthetase (3HPCS), and 3-hydroxypropionyl-CoA dehydratase (3HPCD) Metallosphaera sedula is accompanied by recombinant overexpression of succinyl-CoA synthetase (SCS) from Escherichia coli. The engineered strain produces 13.28 mg/l of acrylic acid. To construct a propionic acid-producing pathway, the same five genes are expressed, with the addition of Metallosphaera sedula acryloyl-CoA reductase (ACR). The engineered strain produces 1430 mg/l of propionic acid, detailed overview |
-, 745491 |
