EC Number |
Protein Variants |
Reference |
---|
1.3.1.124 | D117A |
site-directed mutagenesis, mutant is expressed in inclusion bodies and cannot be refolded to a functional enzyme after extraction from inclusion bodies with 8 M urea as denatured protein |
654376 |
1.3.1.124 | D300A |
site-directed mutagenesis, reduced activity and altered kinetics compared to the wild-type enzyme |
654376 |
1.3.1.124 | E154A |
site-directed mutagenesis, reduced activity and altered kinetics compared to the wild-type enzyme |
654376 |
1.3.1.124 | E227A |
site-directed mutagenesis, reduced activity and altered kinetics compared to the wild-type enzyme |
654376 |
1.3.1.124 | E276A |
site-directed mutagenesis, reduced activity and altered kinetics compared to the wild-type enzyme |
654376 |
1.3.1.124 | E310A |
site-directed mutagenesis, effects on reaction mechanism |
656286 |
1.3.1.124 | E310Q |
site-directed mutagenesis, effects on reaction mechanism |
656286 |
1.3.1.124 | K214A |
site-directed mutagenesis, effects on reaction mechanism |
656286 |
1.3.1.124 | more |
synthesis of polyhydroxyalkanoate from 10-trans-heptadecenoic acid in mutants devoid of 2,4-dienoyl-CoA reductase reveals degradation of the trans fatty acid directly via the enoyl-CoA hydratase II activity of the multifunctional enzyme. Level of polyhydroxyalkanoate is 10-25% to that of wild-type in mutants lacking 2,4-dienoyl-CoA reductase |
-, 661257 |
1.3.1.124 | N148A |
site-directed mutagenesis, effects on reaction mechanism |
656286 |