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EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.21.99.4A131C similar Km-values for L-thyroxine and 3,3',5-triiodothyronine as the wild-type enzyme. Mutation improves the interaction with the reducing cofactor dithiothreitol 658557
Display the word mapDisplay the reaction diagram Show all sequences 1.21.99.4A131S similar Km-values for L-thyroxine and 3,3',5-triiodothyronine as the wild-type enzyme. Mutation improves the interaction with the reducing cofactor dithiothreitol 658557
Display the word mapDisplay the reaction diagram Show all sequences 1.21.99.4more exchange selenocysteine to cysteine by site-directed mutagenesis of isoenzyme type I 639408
Display the word mapDisplay the reaction diagram Show all sequences 1.21.99.4more mutant enzymes, in which selenocysteine residues in the core catalytic center is replaced by cysteine, shows a 10fold increase in Km-value for 3,3',5-triiodo-L-thyronine sulfate. Deletion of the endoplasmic reticulum (ER)-signal sequence and the membrane-spanning domain, amino acids 2-35, does not result in the production of a soluble type I-like enzyme. This mutant protein is inactive but is still membrane-bound 659748
Display the word mapDisplay the reaction diagram Show all sequences 1.21.99.4N203Q decrease in Km-value for 3,3',5-triiodo-L-thyronine sulfate 659748
Display the word mapDisplay the reaction diagram Show all sequences 1.21.99.4N94Q decrease in Km-value for 3,3',5-triiodo-L-thyronine sulfate 659748
Display the word mapDisplay the reaction diagram Show all sequences 1.21.99.4N94Q/N203Q increase in Km-value for 3,3',5-triiodo-L-thyronine sulfate 659748
Display the word mapDisplay the reaction diagram Show all sequences 1.21.99.4P135S the mutant type 2 iodothyronine deiodinase has many type 1 iodothyronine deiodinase-like properties, a Km (L-thyroxine) in the micromolar range, ping-pong kinetics with dithiothreitol, and sensitivity to 6n-propylthiouracil in vitro. When the P135S mutant is expressed in HEK-293 cells and exposed to 2-200 pM free L-thyroxine, the rate of L-thyroxine to 3,5,3'-triiodo-L-thyronine conversion is identical with type 2 iodothyronine deiodinase and conversion is insensitive to 6n-propylthiouracil. Using glutathione as a cofactor in vitro results in a marked decrease in the Km (L-thyroxine) (as also occurs for type 1 iodothyronine deiodinase), it shows sequential kinetics with L-thyroxine and is sensitive to 6n-propylthiouracil but is resistant when HEK-293 cytosol is used as a cofactor 714839
Display the word mapDisplay the reaction diagram Show all sequences 1.21.99.4S101A Km-value for 3,3',5-triiodo-L-thyronine sulfate is nearly identical 659748
Display the word mapDisplay the reaction diagram Show all sequences 1.21.99.4S101A/S176A increase in Km-value for 3,3',5-triiodo-L-thyronine sulfate 659748
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