EC Number   |
Protein Variants |
Reference |
|---|
   1.21.99.3 | E200T |
inactive |
735180, 746292 |
| 1.21.99.3 | more |
generation of enzyme knockout mice |
733734 |
| 1.21.99.3 | R275A |
the mutant shows sharply reduced activity compared to the wild type enzyme |
746292 |
| 1.21.99.3 | S167A |
about 10% residual activity |
735180 |
| 1.21.99.3 | S167A |
the mutant shows about 90% reduced activity compared to the wild type enzyme |
746292 |
| 1.21.99.3 | SeC144A |
site-directed mutagenesis, exchange of the selenocysteine residue in the highly conserved active site sequence, enzymatically inactive |
644808 |
| 1.21.99.3 | SeC144C |
site-directed mutagenesis, exchange of the selenocysteine residue in the highly conserved active site sequence, 5fold increased Km for 3,3',5-triiodo-L-thyronine and 100fold increased Km for 3,3',5,5'-tetraiodo-L-thyronine compared to the wild-type, 2-6fold reduced turnover |
644808 |
| 1.21.99.3 | Sec170C |
mutation in active site, crystallization data |
735180 |
| 1.21.99.3 | T169A |
inactive |
735180, 746292 |
| 1.21.99.3 | T169S |
60% of wild-type activity |
735180 |
