EC Number   |
Protein Variants |
Reference |
|---|
    1.21.4.2 | C242A |
no cleavage on the N-terminal side of a cysteine |
-, 484935 |
| 1.21.4.2 | C242S |
cleavage on the N-terminal side of a cysteine under similar conditions with more extended half-times than wild-type enzyme |
-, 484935 |
| 1.21.4.2 | C242T |
cleavage on the N-terminal side of a cysteine under similar conditions with more extended half-times than wild-type enzyme |
-, 484935 |
| 1.21.4.2 | C353 |
mutation of potentially redox-active motif UxxCxxC, 44% of wild-type peroxidase activity |
671651 |
| 1.21.4.2 | C356 |
mutation of potentially redox-active motif UxxCxxC, 40% of wild-type peroxidase activity |
671651 |
| 1.21.4.2 | C359A |
grdD of protein component C, mutant enzyme completely inactive, accessible to iodoacetate only under native conditions, suggesting that Cys359 of GrdD is the thiol responsible for the formation of the acetyl thioester during catalysis of arsenate-dependent hydrolysis of acetyl phosphate |
636473 |
| 1.21.4.2 | C98S |
grdD of protein component C, activity is unchanged, accessible to iodoacetate only after denaturation |
636473 |
| 1.21.4.2 | more |
mutation of potentially redox-active motif UxxCxxC results in still significant, but decreased peroxidase activity |
671651 |
| 1.21.4.2 | more |
mutation of the potentially redox-active UxxCxxC motif in subunit GrdB of the B protein complex results in still signifiant, but decreased peroxidase activity, overview |
671651 |
| 1.21.4.2 | U350 |
mutation of potentially redox-active motif UxxCxxC, 60% of wild-type peroxidase activity |
671651 |
