EC Number   |
Protein Variants |
Reference |
|---|
    1.2.4.1 | D15A |
site-directed mutagenesis, 0.19% remaining activity with NAD+ and 16% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 |
654734 |
| 1.2.4.1 | D203A |
enzymatic activity similar to wild-type, mutant retains the normal capacity to bind the peripheral subunit-binding domain |
736099 |
| 1.2.4.1 | D276A |
site-directed mutagenesis, reduced activity with pyruvate compared to the wild-type enzyme |
655474 |
| 1.2.4.1 | D289A |
the beta subunit mutant does not have any detectable activity in assays with NAD+ and shows no change in activity in 2,6-dichlorophenolindophenol assays |
686772 |
| 1.2.4.1 | D289N |
the beta subunit mutant shows by 36% reduced activity in assays with NAD+ |
686772 |
| 1.2.4.1 | D549A |
in the mutant, binding and reductive acetylation of E2p lipoyl domains are highly impaired |
725205 |
| 1.2.4.1 | D7A |
site-directed mutagenesis, 0.14% remaining activity with NAD+ and 63% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 |
654734 |
| 1.2.4.1 | D9A |
site-directed mutagenesis, inactive with NAD+, 80% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 |
654734 |
| 1.2.4.1 | E12D |
site-directed mutagenesis, 2.3% remaining activity with NAD+ and 90% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 |
654734 |
| 1.2.4.1 | E12Q |
site-directed mutagenesis, 3.3% remaining activity with NAD+ and 59% reduced E1 activity with 2,6-dichlorophenolindophenol as electron acceptor compared to the wild-type E1 |
654734 |
