EC Number |
Protein Variants |
Reference |
---|
1.2.1.88 | C330A |
site-directed mutagenesis, inactive mutant |
763363 |
1.2.1.88 | D226A |
site-directed mutagenesis, the mutant shows altered kinetics compared to wild-type enzyme |
763363 |
1.2.1.88 | E205A |
site-directed mutagenesis, the mutant shows reduced activity compared to wild-type enzyme |
763363 |
1.2.1.88 | E314A |
inactive. The conserved Glu447 residue has significantly shifted in the mutant, causing NAD+ to be displaced |
740251 |
1.2.1.88 | F202A |
site-directed mutagenesis, the mutant shows reduced activity compared to wild-type enzyme |
763363 |
1.2.1.88 | F505A |
site-directed mutagenesis, the mutant shows reduced activity compared to wild-type enzyme |
763363 |
1.2.1.88 | K104A |
mutation in alpha3 helix which participates in the dimer-dimer interaces. Catalytic efficiency similar to wild-type, mutant forms a hexamer |
-, 740867 |
1.2.1.88 | K329A |
site-directed mutagenesis, the mutant shows reduced activity compared to wild-type enzyme |
763363 |
1.2.1.88 | more |
impairment of pyrroline-5-carboxylate oxidation in the p5cdh mutant does not change the cellular Pro to P5C ratio under ambient and osmotic stress conditions. Lack of P5CDH activity leads to higher ROS production under dark and light conditions in the presence of Pro excess, as well as renders plants hypersensitive to heat stress |
698936 |
1.2.1.88 | more |
mutant strain MB2281-10C with deficiency in enzyme |
392046 |