EC Number   |
Protein Variants |
Reference |
|---|
   1.2.1.80 | C294S |
the mutant avoids spontaneous formation of the acyl-enzyme intermediate |
-, 763498 |
| 1.2.1.80 | K80A |
the mutant does not bind to aldehyde-deformylating oxygenase |
-, 763498 |
| 1.2.1.80 | more |
replacement of the PC7942_orf1593 and 1594 orthologues in Synechocystis sp. PCC6803, where the orthologous genes apparently form only a bicistronic operon, with a kanamycin-resistance cassette. This replacement abolishes the presence of alkanes in the extracts of photoautoptrophically grown cells. PCC7942_orf 1593 and orf1594 coexpression in an Escherichia coli strain devoid of acyl-CoAs leads to very similar levels of hydrocarbon production, comparable to those in an Escherichia coli wild-type strain |
-, 713511 |
| 1.2.1.80 | R73A |
the mutant does not bind to aldehyde-deformylating oxygenase |
-, 763498 |
| 1.2.1.80 | R79A |
the mutant does not bind to aldehyde-deformylating oxygenase |
-, 763498 |
| 1.2.1.80 | Y247A |
the inactive mutant does not bind to aldehyde-deformylating oxygenase |
763498 |
| 1.2.1.80 | Y247F |
the mutant shows strongly increased activity compared to the wild type enzyme |
-, 763498 |
