EC Number |
Protein Variants |
Reference |
---|
1.2.1.5 | C300A |
the variant shows no oxidation activity |
-, 739913 |
1.2.1.5 | C325S |
site-directed mutagenesis, the mutant strain is more sensitive against H2O2 treatment than the wild-type and shows lower NADPH concentration |
-, 762546 |
1.2.1.5 | E140D |
Km-value for NADP+ is about half that of the native enzyme, Km-value for NAD+ is about 3.1fold that of the native enzyme, Km-value for benzaldehyde is about 1.4fold that of the native enzyme, Km-value for propanal is about 1.9fold that of the native enzyme. The coenzyme preference NADP+/NAD+ is 3.2, compared to 3.4 for the native enzyme |
348797 |
1.2.1.5 | E140N |
Km-value for NADP+ is about 10% of that of the native enzyme, Km-value for NAD+ is about 3.2fold that of the native enzyme, Km-value for benzaldehyde is about 1.2fold that of the native enzyme, Km-value for propanal is about 2.3fold that of the native enzyme. The coenzyme preference NADP+/NAD+ is 4.2, compared to 3.4 for the native enzyme |
348797 |
1.2.1.5 | E140Q |
Km-value for NADP+ is about 10% of that of the native enzyme, Km-value for NAD+ is about 7.9fold that of the native enzyme, Km-value for benzaldehyde is about 1.6fold that of the native enzyme, Km-value for propanal is about 1.1fold that of the native enzyme. The coenzyme preference NADP+/NAD+ is 2.8, compared to 3.4 for the native enzyme |
348797 |
1.2.1.5 | E140T |
Km-value for NADP+ is about 20% of that of the native enzyme, Km-value for NAD+ is about 15.3fold that of the native enzyme, Km-value for benzaldehyde is about 60% of that of the native enzyme, Km-value for propanal is about 1.4fold that of the native enzyme. The coenzyme preference NADP+/NAD+ is 5.8, compared to 3.4 for the native enzyme |
348797 |
1.2.1.5 | E194S |
the variant shows 15fold higher catalytic efficiency (kcat/Km) for the reduction of all-trans-retinal than the wild type enzyme |
-, 739913 |
1.2.1.5 | E266A |
the variant shows no oxidation activity |
-, 739913 |
1.2.1.5 | E457V |
the variant shows 7.5fold higher catalytic efficiency (kcat/Km) for the reduction of all-trans-retinal than the wild type enzyme |
-, 739913 |
1.2.1.5 | G192A |
Km-value for NADP+ is about half that of the native enzyme, Km-value for NAD+ is about 70% of that of the native enzyme, Km-value for benzaldehyde is about 90% of that of the native enzyme, Km-value for propanal is about 45% of that of the native enzyme |
348797 |