EC Number |
Protein Variants |
Reference |
---|
1.2.1.48 | C241S |
site-directed mutagenesis, inactive active-site mutant |
743336 |
1.2.1.48 | C244S |
the mutant shows about 90% reduction in catalytic efficiency with benzaldehyde and no activity with 4,4'-diapolycopendial compared to the wild type enzyme |
762884 |
1.2.1.48 | D245N |
NAD+ cosubstrate binding is occuring but catalytic reduction is diminished |
693389 |
1.2.1.48 | E207Q |
site-directed mutagenesis, inactive mutant |
743336 |
1.2.1.48 | E331Q |
site-directed mutagenesis, inactive mutant |
743336 |
1.2.1.48 | F456A |
the mutant shows about 50% reduction in catalytic efficiency with 4,4'-diapolycopendial compared to the wild type enzyme |
762884 |
1.2.1.48 | F456A/F457A |
the mutant shows about 15% reduction in catalytic efficiency with benzaldehyde and no activity with 4,4'-diapolycopendial compared to the wild type enzyme |
762884 |
1.2.1.48 | F457A |
the mutant shows about 40% reduction in catalytic efficiency with 4,4'-diapolycopendial compared to the wild type enzyme |
762884 |
1.2.1.48 | G185A |
disruption of NAD+ binding/utilisation |
693389 |
1.2.1.48 | G412R |
disruption of interaction of side-chains with substrate |
693389 |