EC Number   |
Protein Variants |
Reference |
|---|
   1.17.99.9 | C191A/C197A |
low activity |
758836 |
| 1.17.99.9 | C191A/C197A |
the heme content is reduced to a half of the wild-type level |
759435 |
| 1.17.99.9 | C35A |
low activity |
-, 758836 |
| 1.17.99.9 | C35A/C42A |
heme content of the mutant enzyme is 77% compared to wild-type enzyme |
759435 |
| 1.17.99.9 | C35A/C42A |
no detectable activity |
758836 |
| 1.17.99.9 | DELTA211-217 |
Bacillus subtilis LMT20R is deleted for the ctaA gene and, therefore, completely blocked in heme A synthesis. Plasmid pH216MS is obtained from plasmid pH216M (encoding the mutant CtaA-H216M) and carries a 21 bp deletion in ctaA. The CtaADELTA211-217 variant encoded by pH216MS is active in heme A synthesis and supports assembly of normal levels of cytochrome caa3 |
-, 759191 |
| 1.17.99.9 | E57A |
heme content of the mutant enzyme is 62% compared to wild-type enzyme |
-, 759435 |
| 1.17.99.9 | E57A/H216A |
heme content of the mutant enzyme is 28% compared to wild-type enzyme |
759435 |
| 1.17.99.9 | E57A/H278A |
heme content of the mutant enzyme is 42% compared to wild-type enzyme |
759435 |
| 1.17.99.9 | E57Q |
heme content of the mutant enzyme is 65% compared to wild-type enzyme |
759435 |
