EC Number   |
Protein Variants |
Reference |
|---|
   1.14.99.15 | F185A |
site-directed mutagenesis, the mutant shows activity with cinnamic acid in contrast to the wild-type enzyme |
726728 |
| 1.14.99.15 | F185G |
site-directed mutagenesis, the mutant shows activity with cinnamic acid in contrast to the wild-type enzyme |
726728 |
| 1.14.99.15 | F185I |
site-directed mutagenesis, the mutant shows activity with cinnamic acid in contrast to the wild-type enzyme |
726728 |
| 1.14.99.15 | F185I |
site-directed mutagenesis, the mutation reduces the spin state shift from low- to high-spin on the addition of 4-methoxybenzoate by about 25% compared to the wild-type enzyme, the mutant shows reduced NADH consumption and product formation |
-, 727349 |
| 1.14.99.15 | F185L |
site-directed mutagenesis, the mutant shows activity with cinnamic acid in contrast to the wild-type enzyme, the F185L mutant exhibits 5.5times higher hydroxylation activity for 4-coumaric acid than the wild-type enzyme |
726728 |
| 1.14.99.15 | F185S |
site-directed mutagenesis, the mutant shows reduced activity with 2-naphthoic acid compared to the wild-type enzyme |
726728 |
| 1.14.99.15 | F185T |
site-directed mutagenesis, the mutant shows reduced activity with 2-naphthoic acid compared to the wild-type enzyme |
726728 |
| 1.14.99.15 | F185V |
site-directed mutagenesis, the mutant shows activity with cinnamic acid in contrast to the wild-type enzyme |
726728 |
| 1.14.99.15 | F185V |
site-directed mutagenesis, the mutation reduces the spin state shift from low- to high-spin on the addition of 4-methoxybenzoate by about 35% compared to the wild-type enzyme, the mutant shows reduced NADH consumption and product formation |
-, 727349 |
| 1.14.99.15 | F185W |
site-directed mutagenesis, inactive mutant |
726728 |
