EC Number   |
Protein Variants |
Reference |
|---|
   1.14.14.87 | K375T |
the mutant catalyses the aryl migration from liquiritigenin to yield 3,7,4'-trihydroxyflavanone only, its pH optimum is at pH 6.5 |
660166 |
| 1.14.14.87 | L371V |
inactive |
671696 |
| 1.14.14.87 | L371V/K375T |
the mutant shows 3-5% activity levels compared to the wild type. The mutant produces a mixture of 95% 3beta-hydroxyflavanone and 5% flavone from (2S)-liquiritigenin |
671696 |
| 1.14.14.87 | more |
deletion of the membrane-spanning domain and insertion of a hydrophilic polypeptide in the N-terminus and a four histidine tag at the C-terminus results dramatically enhanced expression in Escherichia coli and and solubility. The purified enzyme exhibits the same activity as the full length membrane-associated enzyme expressed in yeast |
746381 |
| 1.14.14.87 | more |
oestrogen-deficient (ovariectomized, Ovx) mice fed with leaf extract from transgenic plant co-expressing AtMYB12 and GmIFS1 but not wild-type extract exhibit significant conservation of trabecular microarchitecture, reduced osteoclast number and expression of osteoclastogenic genes, higher total serum antioxidant levels, and increased uterine oestrogenicity compared with Ovx mice treated with vehicle (control). The simultaneous coexpression of AtMYB12 and GmIFS1 in tobacco leads to the accumulation of substantial amount of genistein in addition to flavonols |
746013 |
| 1.14.14.87 | S310T |
the mutant also catalyses the aryl migration from liquiritigenin to yield 2,7,4'-trihydroxyisoflavanone, but the ratio of the by-product (3,7,4'-trihydroxyflavanone) formation is increased from 8% to 36% (pH 7.5), and a small amount (7%) of a new product, 7,4'-dihydroxyflavone, is detected compared to the wild type |
660166 |
| 1.14.14.87 | S310T/K375T |
the P450 level of the mutant is approximately 1.5times that of wild type. The mutant shows 3-5% activity levels compared to the wild type |
671696 |
| 1.14.14.87 | S310T/L371V/K375T |
the mutant produces 100% flavone from (2S)-liquiritigenin |
671696 |
| 1.14.14.87 | S310T/L371V/K375T |
the mutant shows 4times higher P450 level than the wild type |
671696 |
