EC Number   |
Protein Variants |
Reference |
|---|
   1.14.14.86 | L387A |
site-directed mutagenesis, the mutant shows 60-70% reduced activity with 16alpha-hydroxy-ent-kaurane compared to the wild-type |
744999 |
| 1.14.14.86 | L387D |
site-directed mutagenesis, the mutation causes the loss of the specific catalytic function from 16alpha-hydroxy-ent-kaurane to 16a-hydroxy-ent-kaurenoic acid |
744999 |
| 1.14.14.86 | L387G |
site-directed mutagenesis, the mutant shows 60-70% reduced activity with 16alpha-hydroxy-ent-kaurane compared to the wild-type |
744999 |
| 1.14.14.86 | L387R |
site-directed mutagenesis, the mutation causes the loss of the specific catalytic function from 16alpha-hydroxy-ent-kaurane to 16alpha-hydroxy-ent-kaurenoic acid |
744999 |
| 1.14.14.86 | L387S |
site-directed mutagenesis, the mutant shows 60-70% reduced activity with 16alpha-hydroxy-ent-kaurane compared to the wild-type |
744999 |
| 1.14.14.86 | L387T |
site-directed mutagenesis, the mutant shows 60-70% reduced activity with 16alpha-hydroxy-ent-kaurane compared to the wild-type |
744999 |
| 1.14.14.86 | more |
enzyme modification by N-terminal deletion of the first 43 or 40 amino acids and replacing with 10 amino acid lysine-rich sequences of MAKKTSSKGK |
744583 |
| 1.14.14.86 | more |
mutation d35Tan-Ginbozu is a single nucleotide substitution located in exon 5, leading to a replacement of arginine by serine |
746089 |
| 1.14.14.86 | more |
usage of a fully codon-optimized construct, along with additional N-terminal deletion and modification, for functional recombinant expression in Escherichia coli, recombinant engineered enzyme is used to carry out 18O2 labelling studies with ent-kaurene, and the intermediates ent-kaurenol and ent-kaurenal, to investigate the multifunctional reaction sequence, overview |
-, 711149 |
