EC Number |
Protein Variants |
Reference |
---|
1.14.14.17 | D335F |
mutation in FAD II binding site, nonfunctional enzyme |
671379 |
1.14.14.17 | D335F |
random mutagenesis, mutation in the FADII site, inactive mutant |
671379 |
1.14.14.17 | D335P |
mutation in FAD II binding site, nonfunctional enzyme |
671379 |
1.14.14.17 | D335P |
random mutagenesis, mutation in the FADII site, inactive mutant |
671379 |
1.14.14.17 | D335W |
mutation in FAD II binding site, nonfunctional enzyme |
671379 |
1.14.14.17 | D335W |
random mutagenesis, mutation in the FADII site, inactive mutant |
671379 |
1.14.14.17 | D407F |
site-directed mutagenesis, mutant shows 8% of wild-type activity |
657764 |
1.14.14.17 | E60A |
site-directed mutagenesis in the highly conserved motif 1, the E60A variant poorly complements growth of KLN1, and shows reduced activity and about 50fold increased sensitivity to terbinafine and naftifine and 5fold to ketoconazole compared to that in the wild type, and confers temperature-sensitive growth |
684491 |
1.14.14.17 | E60Q |
site-directed mutagenesis in the highly conserved motif 1, the E60A variant poorly complements growth of KLN1, and shows highly reduced activity and about 50fold increased sensitivity to terbinafine and naftifine and 5fold to ketoconazole compared to that in the wild type, and confers temperature-sensitive growth |
684491 |
1.14.14.17 | F203A |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
684828 |