EC Number   |
Protein Variants |
Reference |
|---|
    1.14.13.7 | C349S |
the mutant shows 30% activity compared to the wild type enzyme |
743011 |
| 1.14.13.7 | C476S |
the mutant shows 45% activity compared to the wild type enzyme |
743011 |
| 1.14.13.7 | D54N |
slower reaction than wild type enzyme, higher dissociation constant for binding of phenol than wild type enzyme |
438800 |
| 1.14.13.7 | D75N |
the mutant shows 288% activity compared to the wild type enzyme |
743011 |
| 1.14.13.7 | more |
production ToMO mutants with modified regioselectivity compared with the regioselectivity of the wild-type protein in order to alter the ability of the recombinant upper pathway to produce methylcatechol isomers from toluene and to produce 3,4-dimethylcatechol from o-xylene, the combination of ToMO mutant E103G and phenol oxidase increases the production of 4-methylcatechol from toluene and the formation of 3,4-dimethylcatechol from o-xylene, overview |
-, 671422 |
| 1.14.13.7 | P261S |
the mutant shows 15% activity compared to the wild type enzyme |
743011 |
| 1.14.13.7 | P364S |
only 13% of the FAD is utilized to hydroxylate the substrate phenol, when resorcinol is used as substrate, the reaction is not significantly different from the reaction of the wild type enzyme |
438801 |
| 1.14.13.7 | R262S |
the mutant shows 427% activity compared to the wild type enzyme |
743011 |
| 1.14.13.7 | R269L |
the mutant shows 232% activity compared to the wild type enzyme |
743011 |
| 1.14.13.7 | R281M |
slower reaction than wild type enzyme, binds the FAD cofactor more weakly than wild type enzyme |
438800 |
