Any feedback?
Please rate this page
(search_result.php)
(0/150)

BRENDA support

Refine search

Search Protein Variants

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term:

Results 1 - 10 of 10
EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.14.12.24I204L the mutant transforms both 2,6-dinitrotoluene and 2,4-dinitrotoluene 2fold faster than the wild type enzyme and exhibits activity with 2,5- and 2,3-dinitrotoluene -, 733548
Display the word mapDisplay the reaction diagram Show all sequences 1.14.12.24I204Y mutation in alpha subunit DntAc, results in two- to fourfold faster oxidization of the aminonitrotoluenes -, 733549
Display the word mapDisplay the reaction diagram Show all sequences 1.14.12.24I204Y the mutant transforms both 2,6-dinitrotoluene 2,5fold faster than the wild type enzyme and exhibits activity with 2,5- and 2,3-dinitrotoluene -, 733548
Display the word mapDisplay the reaction diagram Show all sequences 1.14.12.24I204Y the mutation results in 2-4fold faster oxidization of the aminonitrotoluenes compared to the wild type enzyme -, 733549
Display the word mapDisplay the reaction diagram Show all sequences 1.14.12.24more construction of hybrid dioxygenases with the genes encoding 2-nitrotoluene 2,3-dioxygenase from Pseudomonas sp. strain JS42 and 2,4-dinitrotoluene dioxygenase from Burkholderia sp. strain DNT. The C-terminal region of the large subunit of the oxygenase component is responsible for the enzyme specificity differences observed between 2-nitrotoluene 2,3-dioxygenase and 2,4-dinitrotoluene dioxygenase -, 734057
Display the word mapDisplay the reaction diagram Show all sequences 1.14.12.24more engineering of hybrid dioxygenase enzymes coexpressing genes from naphthalene and 2,4-dinitrotoluene dioxygenases in Escherichia coli. In the active hybrids, replacement of small subunits affects the rate of product formation but has no effect on the substrate range, regiospecificity, or enantiomeric purity of oxidation products with the substrates tested. The small subunit of the oxygenase is essential for activity but does not play a major role in determining the specificity of these enzymes. Introduction of the small subunit of 2-nitrotolene synthase from Pseudomonas sp. strain JS42 leads to 30% reduction in product formation from naphthalene and dinitrotoluene -, 734058
Display the word mapDisplay the reaction diagram Show all sequences 1.14.12.24V350F mutant displays significantly increased activity towards o-nitrophenol (47 times), m-nitrophenol (34 times), and o-methoxyphenol (174 times) as well as an expanded substrate range that now includes m-methoxyphenol, o-cresol, and m-cresol -, 733152
Display the word mapDisplay the reaction diagram Show all sequences 1.14.12.24V350F the mutant of the alpha subunit is inactive with 2,4-dinitrotoluene but shows significantly increased activity towards 2-nitrophenol (47times), 3-nitrophenol (34times), and 2-methoxyphenol (174times) as well as an expanded substrate range that now includes 3-methoxyphenol, o-cresol, and m-cresol (wild type enzyme has no detectable activity for these substrates). The mutant also exhibits 10fold enhanced activity towards naphthalene forming (1R,2S)-cis-1,2-dihydro-1,2-dihydroxynaphthalene -, 733152
Display the word mapDisplay the reaction diagram Show all sequences 1.14.12.24V350M mutant displays increased activity towards o-nitrophenol (20 times) and o-methoxyphenol (162 times) as well as novel activity towards o-cresol -, 733152
Display the word mapDisplay the reaction diagram Show all sequences 1.14.12.24V350M the mutant is inactive with 2,4-dinitrotoluene but displays increased activity towards 2-nitrophenol (20times) and 2-methoxyphenol (162times) as well as novel activity towards 2-cresol compared to the wild type enzyme. The mutant also exhibits 10fold enhanced activity towards naphthalene forming (1R,2S)-cis-1,2-dihydro-1,2-dihydroxynaphthalene -, 733152
Results 1 - 10 of 10