EC Number |
Protein Variants |
Reference |
---|
1.14.12.11 | D219A |
mutation at alpha-subunit of oxygenase component completely abolishes toluene dioxygenase activity, mutation completely eliminates formation of cis-toluene dihydrodiol |
440344 |
1.14.12.11 | E214A |
mutation at alpha-subunit of oxygenase component completely abolishes toluene dioxygenase activity, mutation completely eliminates formation of cis-toluene dihydrodiol |
440344 |
1.14.12.11 | E609L |
increased activity with all substrates |
658336 |
1.14.12.11 | F366 |
the mutant shows strongly reduced activity compared to the wild type enzyme |
741558 |
1.14.12.11 | H222A |
mutation at alpha-subunit of oxygenase component completely abolishes toluene dioxygenase activity, mutation completely eliminates formation of cis-toluene dihydrodiol |
440344 |
1.14.12.11 | H228A |
mutation at alpha-subunit of oxygenase component completely abolishes toluene dioxygenase activity, mutation completely eliminates formation of cis-toluene dihydrodiol |
440344 |
1.14.12.11 | I324F |
the mutant shows reduced activity compared to the wild type enzyme |
741558 |
1.14.12.11 | more |
Escherichia coli expressed mutant enzyme TDO 2-B38, in which the wild-type stop codon is replaced with a codon encoding threonine, exhibits 5.6times higher activity towards 4-picoline and about 20% more activity towards toluene than the wild-type enzyme |
440352 |
1.14.12.11 | more |
introduction of a plasmid containing the entire tod operon todC1C2BADE to Pseudomonas putida T57 to enhance its ability to degrade 4-chloroaniline. The resulting strain shows 250-fold higher 4-chloroaniline degradation activity the parental strain. The recobinant strain degrades 2-chloroaniline, 3-chloroaniline, and 3,4-dichloroaniline as well as 3-chloroaniline, but not 3,5-dichloroaniline |
-, 725577 |
1.14.12.11 | more |
mutants SP6-1A, SP6-1G, and SP14-4H (created by error-prone PCR mutagenesis) show decreased 1-indenol production in comparison to the wild type enzyme |
681757 |