EC Number   |
Protein Variants |
Reference |
|---|
  1.14.11.48 | C357A |
enhanced activity towards 9-methylxanthine as compared to wild-type enzyme. Mutant enzyme is resistant rto thiol-specific reagents that inactivated wild-type enzyme. Elevated ferroxidase activity in the absence of substrates |
731217 |
| 1.14.11.48 | D138A |
enhanced activity towards 9-methylxanthine as compared to wild-type enzyme. Significant decrease in Ki(app) for 6,8-dihydroxypurine |
731217 |
| 1.14.11.48 | D151A |
inactive mutant enzyme |
731217 |
| 1.14.11.48 | E137A |
enhanced activity towards 9-methylxanthine as compared to wild-type enzyme. Significant decrease in Ki(app) for 6,8-dihydroxypurine |
731217 |
| 1.14.11.48 | H149A |
inactive mutant enzyme |
731217 |
| 1.14.11.48 | H340A |
mutant enzyme exhibits 0.17% of the wild-type enzyme activity |
731217 |
| 1.14.11.48 | K122A |
2fold increase in the Kd of 2-oxoglutarate compared to wild-type enzyme |
731217 |
| 1.14.11.48 | N358A |
23fold decrease in kcat/Km compared to wild-type enzyme. Significant decrease in Ki(app) for 6,8-dihydroxypurine |
731217 |
| 1.14.11.48 | Q101A |
elevated ferroxidase activity in the absence of substrates |
731217 |
| 1.14.11.48 | Q356A |
significant decrease in Ki(app) for 6,8-dihydroxypurine. Elevated ferroxidase activity in the absence of substrates |
731217 |
