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EC Number
Protein Variants
Commentary
Reference
1.14.11.32
E338G/I340L/L341V/K342E
site-directed mutagenesis, the mutant exhibits demethylase activity exclusively toward codeine, not also toward thebaine like the wild-type enzyme
724726
1.14.11.32
R334S/R336S
site-directed mutagenesis, the mutant retains O-demethylase activity toward either thebaine or codeine like the wild-type enzyme
724726
1.14.11.32
R334S/R336T
site-directed mutagenesis, the mutant retains O-demethylase activity toward either thebaine or codeine like the wild-type enzyme
724726
1.14.11.32
S149M/L151F/S152T
site-directed mutagenesis, the mutant is expressed at low levels, perhaps due to improper folding
724726
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