EC Number   |
Protein Variants |
Reference |
|---|
   1.13.99.1 | D82Y |
the mutant displays significantly decreased Km values and better kcat value compared to the wild type enzyme |
765322 |
| 1.13.99.1 | DELTA miox 1+2 |
double mutant, smaller syncytia, less female nematodes per plant |
700488 |
| 1.13.99.1 | delta miox 4+5 |
double mutant, smaller syncytia, less female nematodes per plant |
700488 |
| 1.13.99.1 | DELTA miox1 |
single knockout mutant of one allele of MIOX |
700488 |
| 1.13.99.1 | delta miox2 |
single knockout mutant of one allele of MIOX |
700488 |
| 1.13.99.1 | delta miox5 |
single knockout mutant of one allele of MIOX |
700488 |
| 1.13.99.1 | L240M |
the mutant's activity shows a drastic improvement compared with the wild type enzyme |
765322 |
| 1.13.99.1 | more |
coexpression of the Mus musculus mMIOX and the urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 as fusion protein leads highly increased glutaric acid levels from myo-inositol in transfected Pichia pastoris cells. Production of glucaric acid with a fed-batch fermentation strategy, overview. Recombinant coexpression of the enzyme in Escherichia coli strains BL21(DE3) or MG1655 with myo-inositol-1-phosphate synthase from Saccharomyces cerevisiae and urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 leads to highly increased glutaric acid production, especially with addition of myo-inositol instead of glucose, production parameters, overview |
744811 |
| 1.13.99.1 | more |
construction of a quadruple (miox1/2/4/5) mutant by mutation of all four MIOX isozymes via T-DNA insertion, metabolite profiling of syncytia induced in roots of the myo-inositol oxygenase quadruple mutant, overview. The quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. The miox1/2/4/5 plants show a similar reduction in the number of females to miox double mutants |
-, 745898 |
| 1.13.99.1 | more |
generation of an AtMIOX1 gene T-DNA insertion mutant atmiox1, the AtMIOX1 gene is efficiently silenced by the T-DNA insertion in the atmiox1 mutant. Constitutive overepression of Glycine soja MIOX1 under constitutive CaMV35S promoter control can recover the phenotype leading to alkaline stress tolerance in the atmiox1 mutant plants, overview |
746190 |
