EC Number |
Protein Variants |
Reference |
---|
1.13.12.9 | K478A |
catalytic activity 1/200 of the wild-type enzyme |
-, 698757 |
1.13.12.9 | M142A |
kcat is 1/10 of the wild-type enzyme, Km is elevated, affinity for oxygen seems decreased |
-, 698757 |
1.13.12.9 | R143A |
inactive |
-, 727793 |
1.13.12.9 | R143A |
mutant enzyme shows no activity |
-, 727793 |
1.13.12.9 | R143K |
mutant enzyme shows 400fold lower activity compared to wild-type. Wild-type PAOpt produces higher amounts of phenylacetamide (PAM) than phenylpyruvate (PPV) as expected, but PAM is not produced by the R143K and Y536F mutants |
-, 727793 |
1.13.12.9 | R143K |
the mutant has about 400fold lower activity than the wild type enzyme |
-, 727793 |
1.13.12.9 | Y536A |
inactive |
727793 |
1.13.12.9 | Y536A |
mutant enzyme shows no activity |
727793 |
1.13.12.9 | Y536F |
mutant enzyme shows 17fold lower activity compared to wild-type. Wild-type PAOpt produces higher amounts of phenylacetamide (PAM) than phenylpyruvate (PPV) as expected, but PAM is not produced by the R143K and Y536F mutants |
-, 727793 |
1.13.12.9 | Y536F |
the mutant has about 17fold lower activity than the wild type enzyme |
727793 |